Superior Cytokine Boost:

Human mesenchymal stem cells (MSCs) cultured in vitro secrete a complex mixture of bioactive molecules, including soluble factors (e.g., growth factors and cytokines) and extracellular vesicles, collectively referred to as the secretome. Discovery of the secretome’s regenerative and immunomodulatory properties has led to further investigation into its potential use in cell-free therapeutic approaches. Our proprietary MSC secretome significantly upregulated nine key cytokines, which enhance tissue regeneration via angiogenesis and collagen production, while eliminating inflammatory CXCL7/NAP-2 for safer outcomes.

Faster Wound Healing:

The cell migration assay is an in vitro method used to study the wound-healing process. It enables quantification of cell movements in response to treatment with biologically active compounds, providing insight into their potential efficacy in vivo. Our data demonstrate that the secretome collected from MSCs cultured using InterceptAge’s proprietary method is 2.6-fold more effective in promoting the migration of human skin fibroblasts in vitro compared to that obtained using the conventional method.

Cellular senescence is a major driver of aging, typically triggered by oxidative stress. Senescence-associated beta-galactosidase (SA-β-gal) serves as a well-established biomarker for cellular aging. In an oxidative stress-induced senescence model using primary human dermal fibroblasts, InterceptAge secretome demonstrated potent protective activity by significantly reducing SA-β-gal expression. This performance substantially exceeded that of conventional secretomes, establishing a strong foundation for its application in advanced anti-aging treatments.

Prevention of senescence:

InterceptAge secretome strongly reduces the level of senescent cells

SA-β-Gal Staining of Senescent Cells

Modulation of stress fiber formation:

During aging, dermal fibroblasts can adopt a persistently activated myofibroblast-like phenotype characterized by prominent stress fibers that maintain chronic contractile tension. This sustained mechanical stress accelerates and deepens wrinkle formation, particularly in regions subjected to repeated facial muscle contractions

To investigate the capacity of InterceptAge secretome to modulate stress fiber formation, we induced stress fibers in primary human dermal fibroblasts and subsequently treated these cells with either InterceptAge secretome or conventional MSC secretome. Immunofluorescence staining for alpha-smooth muscle actin (α-SMA) was performed to visualize stress fiber formation. Our analysis revealed that InterceptAge secretome significantly outperformed conventional MSC secretome in reducing stress fiber content in human dermal fibroblasts. These findings suggest that InterceptAge secretome may help restore balanced fibroblast function, thereby facilitating cellular rejuvenation.

Preservation of Secretome Bioactivity:

Lyophilization is essential for the long-term storage of the secretome, as it preserves stability at ambient temperatures, eliminates the need for cold-chain logistics and significantly extends shelf life (months, compared to weeks for liquid formulations). Relative to the control treatment of human dermal fibroblasts with secretome-free medium, the frozen/thawed IntercepAge MSC secretome increased the cell viability by approximately 2.5-fold. Post-lyophilization evaluation further demonstrated that the secretome retained its biological activity, as indicated by fibroblast viability comparable to that observed with the frozen/thawed control.